This article contains a list of the most studied restriction enzymes whose names start with Bd to Bp inclusive. It contains approximately 100 enzymes.

The following information is given:

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Restriction enzymes

Bd - Bp

Enzyme PDB code Source Recognition sequence Cut IsoschizomersBdiI Brevibacterium divaricatum 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BavCI, Bci29I, Bli41I, Bli86I, BseCI, BsuTUI, Rme21I, SpmIBdiSI Bacteroides distasonis 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' BecAII Brevibacterium sp. A 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BepI Brevibacterium epidermidis 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BepI, BspFNI, BstUI, Bsu1532I, BtkI, FalII, FauBII, ThaIBetI Brevibacterium acetyliticum 5' WCCGGW 3' WGGCCW 5' ---W CCGGW--- 3' 3' ---WGGCC W--- 5' BfaI Bacteroides fragilis 5' CTAG 3' GATC 5' ---C TAG--- 3' 3' ---GAT C--- 5' BfiI 2C1L Bacillus firmus S8120 5' ACTGGG 3' TGACCC 5' ---ACTGGGNNNNN --- 3' 3' ---TGACCCNNNN N--- 5' BmrIBfi57I Butyrivibrio fibrisolvens OB157 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bsp105I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, SsiBIBfi89I Butyrivibrio fibrisolvens OB189 5' YGGCCR 3' RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' BflI Bacillus flavothermus 5' CCN7GG 3' GGN7CC 5' ---CCNNNNN NNGG--- 3' 3' ---GGNN NNNNNCC--- 5' BfmI Bacillus firmus S8-336 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' BfrI Bacteroides fragilis 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' BfrBI Bacteroides fragilis BE3 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' Csp68KIII, EcoT22I, Mph1103I, PinBI, Ppu10I, SepI, SspD5II, Zsp2IBfuI Bacillus firmus Auk 22-m21 5' GTATCC 3' CATAGG 5' ---GTATCCN4NN --- 3' 3' ---CATAGGN4N N--- 5' BciVIBfuAI Bacillus fusiformis 5' ACCTGC 3' TGGACG 5' ---ACCTGCNNNN NNNN--- 3' 3' ---TGGACGNNNNNNNN --- 5' Acc36I, BspMI, BveIBfuCI Bacillus fusiformis 1226 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, MkrAI, Sth368IBglI 1DMU Bacillus globigii 5' GCCN5GGC 3' CGGN5CCG 5' ---GCCNNNN NGGC--- 3' 3' ---CGGN NNNNCCG--- 5' BglII 1ES8 Bacillus globigii 5' AGATCT 3' TCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5' BimI Brevibacterium immotum 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, AsuII, Bpu14I, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuIBim19I Brevibacterium immotum 19 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, AsuII, Bpu14I, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuIBim19II Brevibacterium immotum 19 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BinI Bifidobacterium infantis 659 5' GGATC 3' CCTAG 5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' AclWI, AlwI, BsrWI, BspPI, BstH9I, Bth617I, EacIBlfI Bacillus licheniformis 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, Aor13HI, BseAI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBIIBli41I Bacillus licheniformis 41 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' Bci29I, Bli86I, BseCI, BsiXI, BsuTUI, Rme21I, SpmI, Ssp27144IBli86I Bacillus licheniformis 86 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BliAI, Bli41I, BseDI, BsiXI, Rme21I, SpmI, Ssp27144I, ZhoIBli736I Bacillus licheniformis 736 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' BliAI Bacillus licheniformis 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BliRI, Bli41I, BsiXI, BspDI, Rme21I, SpmI, Ssp27144I, ZhoIBliHKI Bacillus licheniformis HK 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAIBliRI Bacillus licheniformis 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BciBI, BdiI, Bli41I, BsiXI, BspDI, BspXI, LplI, SpmI, ZhoIBlnI Brevibacterium linens 5' CCTAGG 3' GGATCC 5' ---C CTAGG--- 3' 3' ---GGATC C--- 5' AspA2I, AvrII, AvrBII, BspA2I, XmaJIBloHI Bifidobacterium longum E194b 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' BloHII Bifidobacterium longum E194b 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, Asp713I, BsuBI, CflI, CfuII, HalII, PstI, SalPI, SflI, Sst12IBlpI Bacillus lentus 5' GCTNAGC 3' CGANTCG 5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' BluI Brevibacterium luteum 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BssHI, BstVI, PanI, Sau3239I, Sfr274I, TliI, XhoIBme12I Bacillus megaterium 12 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, SsiBIBme18I Bacillus megaterium 18 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinIBme142I Bacillus megaterium 142 5' RGCGCY 3' YCGCGR 5' ---RGC GCY--- 3' 3' ---YCG CGR--- 5' AccB2I, BfoI, Bsp143II, BstH2I, HaeII, LpnIBme216I Bacillus megaterium 216 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, Csp68KI, Eco47I, FssI, HgiCII, HgiJI, SinIBme361I Bacillus megaterium 361 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' Bme585I Bacillus mesentericus 585 5' CCCGC 3' GGGCG 5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5' Bme1390I Bacillus megaterium RFL1390 5' CCNGG 3' GGNCC 5' ---CC NGG--- 3' 3' ---GGN CC--- 5' Bme1580I Bacillus megaterium 1580 5' GKGCMC 3' CMCGKG 5' ---GKGCM C--- 3' 3' ---C MCGKG--- 5' BmgBI Bacillus megaterium 5' CACGTC 3' GTGCAG 5' ---CAC GTC--- 3' 3' ---GTG CAG--- 5' BmrI Bacillus megaterium 5' ACTGGG 3' TGACCC 5' ---ACTGGGNNNNN --- 3' 3' ---TGACCCNNNN N--- 5' BfiIBmtI Bacillus megaterium S2 5' GCTAGC 3' CGATCG 5' ---GCTAG C--- 3' 3' ---C GATCG--- 5' AceII, AsuNHI, BspOI, NheI, LlaG2I, PstNHIBmyI Bacillus mycoides 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduIBnaI Bacillus natto B3364 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, BamHI, Bce751I, BstI, CelI, OkrAI, Nsp29132II, Pfl8I, SolIBoxI Brevibacterium oxydans Iti 12-025 5' GACN4GTC 3' CTGN4CAG 5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5' BpcI Bacillus sphaericus 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' BpiI Bacillus pumilus sw 4-3 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BplI Bacillus pumilus 5' GAGN5CTC 3' CTCN5GAG 5' ---GAGN5CTCN7NNNNNN --- 3' 3' ---CTCN5GAGN7N NNNNN--- 5' BpmI Bacillus pumilus 5' CTGGAG 3' GACCTC 5' ---CTGGAGN13NNN --- 3' 3' ---GACCTCN13N NN--- 5' BpoAI Bacillus polymyxa 5' ATTAAT 3' TAATTA 5' ---AT TAAT--- 3' 3' ---TAAT TA--- 5' AseI, AsnI, PshBI, Sru4DI, VspIBptI Bacillus pantothenticus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, BseBI, Bse16I, BstNI, BstOI, BstM6I, Bst2UI, EcoRII, MvaIBpuI Bacillus pumilus AHU1387A 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' Bpu10I Bacillus pumilus 10 5' CCTNAGC 3' GGANTCG 5' ---CC TNAGC--- 3' 3' ---GGANT CG--- 5' Bpu14I Bacillus pumilus 14 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, AsuII, BsiCI, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuIBpu95I Bacillus pumilus 95 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BepI, BspFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaIBpu1102I Bacillus pumilus RFL1102 5' GCTNAGC 3' CGANTCG 5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' BpuAI Bacillus pumilus 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BpuAmI Bacillus pumilus 5' GAGCTC 3' CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5' BpuB5I Bacillus pumilus 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' BpuDI Bacillus pumilus 1117 5' CCTNAGC 3' GGANTCG 5' ---CC TNAGC--- 3' 3' ---GGANT CG--- 5' BpuEI Bacillus pumilus 2187a 5' CTTGAG 3' GAACTC 5' ---CTTGAGN13NNN --- 3' 3' ---GAACTCN13N NN--- 5' BpuJI 2VLA Bacillus pumilus RFL1458 5' CCCGT 3' GGGCA 5' ---CCCGTN10N NNNNNNNN--- 3' 3' ---GGGCAN10NNNNNNNNN --- 5' — None in May 2010 —BpuSI Bacillus pumilus 5' GGGAC 3' CCCTG 5' ---GGGACN9N NNNN--- 3' 3' ---CCCTGN9NNNNN --- 5'

Notes