List of restriction enzyme cutting sites: E–F

This article contains a list of the most studied restriction enzymes whose names start with E to F inclusive. It contains approximately 110 enzymes.

The following information is given:

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Restriction enzymes

E

Enzyme PDB code Source Recognition sequence Cut IsoschizomersEacI Eubacterium acidaminophilum 5' GGATC 3' CCTAG 5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' AclWI, AlwI, BinI, BpuFI, BspPI, BstH9I, Bst31TI, Bth617I, Ral8IEaeI Enterobacter aerogenes 5' YGGCCR 3' RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' Eae46I Enterobacter aerogenes 46 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' EaeAI Enterobacter aerogenes 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EclRI, PaeBI, PspAI, PspALI, XcyI, XmaI, XmaCIEagI Enterobacter agglomerans 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIIIEagBI Enterobacter agglomerans CBNU45 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorIIEagMI Enterobacter agglomerans M3 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEIEam1104I Enterobacter amnigenus RFL1104 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' Eam1105I Enterobacter amnigenus RFL1105 5' GACN5GTC 3' CTGN5CAG 5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' AhdI, AspEI, BmeRI, BspOVI, DriI, EclHKI, NruGIEarI Enterobacter aerogenes 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' EcaI Enterobacter cloacae 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128IEciI Escherichia coli 5' GGCGGA 3' CCGCCT 5' ---GGCGGAN8NNN --- 3' 3' ---CCGCCTN8N NN--- 5' Eci125I Escherichia coli VKM-125 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128IEclI Enterobacter cloacae 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' Ecl136II Enterobacter cloacae RFL136 5' GAGCTC 3' CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5' EclHKI Enterobacter cloacae 5' GACN5GTC 3' CTGN5CAG 5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' AhdI, AspEI, BmeRI, BspOVI, DriI, Eam1105I, NruGIEclRI Enterobacter cloacae R 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI, XcyI, XmaI, XmaCIEclXI Enterobacter cloacae 590 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIIIEcl18kI 2FQZ Enterobacter cloacae 18k 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' Ecl37kI Enterobacter cloacae 37k 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenIEcl2zI Enterobacter cloacae 2z 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenIEco24I Escherichia coli RFL24 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' Eco31I Escherichia coli RFL31 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' Eco32I Escherichia coli RFL32 5' GATATC 3' CTATAG 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' Eco47I Escherichia coli RFL47 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEIEco47III Escherichia coli RFL47 5' AGCGCT 3' TCGCGA 5' ---AGC GCT--- 3' 3' ---TCG CGA--- 5' AfeI, AitI, Aor51H, FunIEco52I Escherichia coli RFL52 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIIIEco56I Escherichia coli RFL56 5' GCCGGC 3' CGGCCG 5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5' Eco57I Escherichia coli RFL57 5' CTGAAG 3' GACTTC 5' ---CTGAAGN12NNNN --- 3' 3' ---GACTTCN12NN NN--- 5' AcuI, BspKT5IEco64I Escherichia coli RFL64 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAIEco72I Escherichia coli RFL72 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCIEco78I Escherichia coli RFL78 5' GGCGCC 3' CCGCGG 5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5' Eco81I Escherichia coli RFL81 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAIEco88I Escherichia coli RFL88 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAIEco91I Escherichia coli RFL91 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAIIEco105I Escherichia coli RFL105 5' TACGTA 3' ATGCAT 5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5' Eco130I Escherichia coli RFL130 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' Eco147I Escherichia coli RFL147 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuIEco255I Escherichia coli RFL255 5' AGTACT 3' TCATGA 5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5' Acc113I, AssI, BmcAI, Bpa34I, DpaI, RflFII, ScaI, ZrmIEco1831I Escherichia coli RFL1831 5' CCSGG 3' GGSCC 5' --- CCSGG--- 3' 3' ---GGSCC --- 5' AhaI, AseII, AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22IEcoA4I Escherichia coli A4 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' EcoHI Escherichia coli HI 5' CCSGG 3' GGSCC 5' --- CCSGG--- 3' 3' ---GGSCC --- 5' AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22I, Kpn49kII, NciIEcoHK31I Escherichia coli HK31 5' YGGCCR 3' RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' EcoICRI Escherichia coli 2bT 5' GAGCTC 3' CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5' Eco75KI Escherichia coli BKM 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' Eco57MI Escherichia coli RFL57M 5' CTGRAG 3' GACYTC 5' ---CTGRAGN12NNNN --- 3' 3' ---GACYTCN12NN NN--- 5' EcoNI Escherichia coli 5' CCTN5AGG 3' GGAN5TCC 5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5' EcoO44I Escherichia coli O44 Hiromi 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' EcoO65I Escherichia coli K11a 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, Bse64I, BstEII, BstPI, BstT9I, EcaI, Eco91I, EcoO128IEcoO109I 1WTD Escherichia coli H709c 5' RGGNCCY 3' YCCNGGR 5' ---RG GNCCY--- 3' 3' ---YCCNG GR--- 5' EcoO128I Escherichia coli O128Ly3 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AspAI, Bse64I, BstEII, BstT9I, EcaI, EcoO65I, NspSAII, PspEIEcoP15I Escherichia coli P15 5' CAGCAG 3' GTCGTC 5' ---CAGCAGN25NN --- 3' 3' ---GTCGTCN25 NN--- 5' EcoR124II , Search Escherichia coli 5' 5'-GAAN7RTCG-3' 5' EcoRI 1QC9, Search EcoR1 structures Escherichia coli RY13 5' GAATTC 3' CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' EcoRII 1NA6 Escherichia coli R245 5' CCWGG 3' GGWCC 5' --- CCWGG--- 3' 3' ---GGWCC --- 5' ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI, ZanIEcoRV 1RVE Search EcoRV Escherichia coli J62 pLG74 5' GATATC 3' CTATAG 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' EcoT14I Escherichia coli TB14 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' EcoT22I Escherichia coli TB22 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5IIEcoT38I Escherichia coli TH38 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' EcoVIII Escherichia coli E1585-68 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' Eco13kI Escherichia coli 13k 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' Eco21kI Escherichia coli 21k 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' Eco27kI Escherichia coli 27 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAIEco29kI Escherichia coli 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Eco53kI Escherichia coli 53k 5' GAGCTC 3' CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5' Eco137kI Escherichia coli 137k 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' EgeI Enterobacter gergoviae NA 5' GGCGCC 3' CCGCGG 5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5' EheI Erwinia herbicola 9/5 5' GGCGCC 3' CCGCGG 5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5' ErhI Erwinia rhaponici B9 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' ErhB9I Erwinia rhaponici B9 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorIIErhB9II Erwinia rhaponici B9 5' CCWWGG 3' GGWWCC 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' ErpI Erwinia rhaponici 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEIEsaBC3I Environmental sample BC3 5' TCGA 3' AGCT 5' ---TC GA--- 3' 3' ---AG CT--- 5' EsaBC4I Environmental sample BC4 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' EspI Eucapsis sp. 5' GCTNAGC 3' CGANTCG 5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' Esp3I Erwinia sp. RFL3 5' CGTCTC 3' GCAGAG 5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5' Esp4I Erwinia sp. RFL4 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' Esp1396I Enterobacter sp. RFL1396 5' CCAN5TGG 3' GGTN5ACC 5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I

F

Enzyme PDB code Source Recognition sequence Cut IsoschizomersFalI Flavobacterium aquatile Ob10 5' AAGN5CTT 3' TTCN5GAA 5' ---AAGN5CTTN7NNNNNN --- 3' 3' ---TTCN5GAAN7N NNNNN--- 5' FalII Flavobacterium aquatile Ob10 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI, ThaIFaqI Flavobacterium aquatile RFL1 5' GGGAC 3' CCCTG 5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' FatI Flavobacterium aquatile NL3 5' CATG 3' GTAC 5' --- CATG--- 3' 3' ---GTAC --- 5' FauI Flavobacterium aquatile 5' CCCGC 3' GGGCG 5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5' FauBII Flavobacterium aureus B 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI, ThaIFauNDI Flavobacterium aquatile ND 5' CATATG 3' GTATAC 5' ---CA TATG--- 3' 3' ---GTAT AC--- 5' FbaI Flavobacterium balustinum 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BclI, BsiQI, BspXII, BstT7I, Ksp22I,