N-carbamoyl-L-amino-acid hydrolase () is an enzyme that catalyzes the general chemical reaction:
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H2O L-2-amino acid + NH3 + CO2
For example, the enzyme characterised from Sinorhizobium meliloti hydrolyses carglumic acid to L-glutamic acid, with ammonia and carbon dioxide as byproducts:
This reaction is part of biochemical pathway called the hydantoinase process which converts 5-substituted hydantoins into optically pure D- or L-amino acids.
This enzyme is a hydrolase which act on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-carbamoyl-L-amino acid amidohydrolase.
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