N-carbamoylsarcosine amidase () is an enzyme characterised from Pseudomonas putida that catalyzes the hydrolysis of 3-methylhydantoic acid (N-carbamoylsarcosine) to sarcosine, carbon dioxide, and ammonia:
The reaction is part of the breakdown pathway for creatinine.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-carbamoylsarcosine amidohydrolase. This enzyme is also called carbamoylsarcosine amidase.
Structural studies As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .
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