An α,α-trehalase () is an enzyme with systematic name α,α-trehalose glucohydrolase. This enzyme catalyzes the chemical reaction
The reaction gives at first equimolar amounts of α- and β-D-glucose. The enzyme is a hydrolase, specifically a glycosidase that hydrolyses O- and S-glycosyl compounds. It is also called trehalase, and it participates in starch and sucrose metabolism.
Structural studies As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .
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