Beta-aspartyl-N-acetylglucosaminidase () is an enzyme that catalyzes the chemical reaction

The enzyme characterised from epididymis hydrolyses aspartylglucosamine to N-acetyl-D-glucosamine and L-asparagine. The enzyme also acts on other nitrogen-linked glycans.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is 1-beta-aspartyl-N-acetyl-D-glucosaminylamine L-asparaginohydrolase. This enzyme is also called beta-aspartylacetylglucosaminidase.

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