The following highways are numbered 53:
Cambodia National Road 53 (Cambodia)
Canada Alberta Highway 53
Greece EO53 road
India National Highway 53 (India)
Ireland N53 road (Ireland)
Italy Autostrada A53
Japan Japan National Route 53
Korea, South National Route 53 (South Korea)
Mexico Mexican Federal Highway 53
New Zealand New Zealand State Highway 53
Norway (, )
Philippines N53 highway (Philippines)
Turkey , a motorway in Turkey connecting with İskenderun, Hatay Province.
United Kingdom British A53 (Shrewsbury-Buxton) British M53 (Wallasey-Chester)
United States Interstate 53 (former proposal) U.S. Route 53 Alabama State Route 53 Arkansas Highway 53 California State Route 53 Colorado State Highway 53 Connecticut Route 53 Florida State Road 53 County Road 53 (Lafayette County, Florida) County Road 53 (Madison County, Florida) Georgia State Route 53 Idaho State Highway 53 Illinois Route 53 Indiana State Road 53 Iowa Highway 53 (1926-1949) (former) K-53 (Kansas highway) Kentucky Route 53 Louisiana Highway 53 Louisiana State Route 53 (former) Maryland Route 53 Massachusetts Route 53 M-53 (Michigan highway) County Road 53 (Anoka County, Minnesota) County Road 53 (Hennepin County, Minnesota) County Road 53 (Ramsey County, Minnesota) Mississippi Highway 53 Missouri Route 53 Nebraska Highway 53 Nebraska Link 53A Nebraska Link 53B Nebraska Link 53C Nebraska Link 53E Nevada State Route 53 (former) New Jersey Route 53 County Route 53 (Bergen County, New Jersey) County Route 53 (Monmouth County, New Jersey) County Route 53 (Ocean County, New Jersey) New Mexico State Road 53 New York State Route 53 County Route 53 (Cattaraugus County, New York) County Route 53 (Chautauqua County, New York) County Route 53 (Delaware County, New York) County Route 53 (Dutchess County, New York) County Route 53 (Franklin County, New York) County Route 53 (Greene County, New York) County Route 53 (Jefferson County, New York) County Route 53 (Madison County, New York) County Route 53 (Monroe County, New York) County Route 53 (Montgomery County, New York) County Route 53 (Oneida County, New York) County Route 53 (Onondaga County, New York) County Route 53A (Onondaga County, New York) County Route 53 (Orange County, New York) County Route 53 (Orleans County, New York) County Route 53 (Otsego County, New York) County Route 53 (Putnam County, New York) County Route 53 (Rensselaer County, New York) County Route 53 (Rockland County, New York) County Route 53 (Saratoga County, New York) County Route 53 (Suffolk County, New York) County Route 53 (Sullivan County, New York) County Route 53 (Ulster County, New York) County Route 53A (Ulster County, New York) County Route 53 (Warren County, New York) County Route 53 (Westchester County, New York) County Route 53 (Wyoming County, New York) North Carolina Highway 53 North Dakota Highway 53 Ohio State Route 53 Oklahoma State Highway 53 Oklahoma State Highway 53A Oregon Route 53 Pennsylvania Route 53 South Carolina Highway 53 South Dakota Highway 53 Tennessee State Route 53 Texas State Highway 53 Texas State Highway Spur 53 Farm to Market Road 53 (former) Texas Park Road 53 (former) Utah State Route 53 Vermont Route 53 Virginia State Route 53 West Virginia Route 53 West Virginia Route 53 (1920s) (former) Wisconsin Highway 53 (former)
Territories: Puerto Rico Highway 53