Route 250, or Highway 250, may refer to:
Canada Manitoba Provincial Road 250 Prince Edward Island Route 250
Costa Rica National Route 250
Japan Japan National Route 250
United Kingdom road B250 road
United States U.S. Route 250 Arkansas Highway 250 California State Route 250 Georgia State Route 250 (former) Hawaii Route 250 Illinois Route 250 Indiana State Road 250 Kentucky Route 250 Minnesota State Highway 250 Montana Secondary Highway 250 Nebraska Highway 250 New Mexico State Road 250 New York State Route 250 Oregon Route 250 Pennsylvania Route 250 (former) Tennessee State Route 250 Texas State Highway 250 (former) Texas State Highway Loop 250 Farm to Market Road 250 (Texas) Utah State Route 250 (former) Territories: Puerto Rico Highway 250