E16, E-16, E.16 or E 16 may refer to:
Computing The version 0.16 of the Enlightenment window manager
Military Aichi E16A, an Imperial Japanese Navy seaplane which saw service during World War II HMS E16, a United Kingdom Royal Navy submarine which saw service during World War I
Transportation European route E16 County Route E16 (California), a county route in El Dorado County, California, United States The FAA location identifier for San Martin Airport in San Martin, California The DRG Class E 16, a German electric locomotive Yokohama–Yokosuka Road, an expressway numbered E16 in Japan
Other E16, a postcode district in the E postcode area of London E16 is sometimes used to abbreviate Echovirus 16, the cause of Boston exanthem disease Alien of Extraordinary Ability (EB-1A, officially E11 / E16), a special green card for high achieving immigrants in United States.