Charlotte Harbor may refer to:
Charlotte Harbor, Florida, a census-designated place in Charlotte County, Florida, United States Charlotte Harbor (estuary), a large bay on the southwest coast of Florida Charlotte Harbor Light, a former lighthouse in Charlotte Harbor Charlotte Harbor Preserve State Park, a Florida State Park composed of islands and land that surround the Charlotte Harbor estuary Charlotte Harbor and Northern Railway, a former railroad in southwest Florida Charlotte Harbor and Northern Railway Depot, a historic railroad depot in Boca Grande, Florida