There are 300 American, Canadian, and Puerto Rican colleges and universities classified as Division II for NCAA competition during the 2026–27 academic year, including four schools that are in the process of reclassifying to Division II. Forty-four of the 50 U.S. states, plus the District of Columbia, the commonwealth of Puerto Rico, and the Canadian province of British Columbia are represented. Arizona, Louisiana, Maine, Nevada, Rhode Island, and Wyoming do not currently have D-II institutions, although a Louisiana-based institution has announced plans to transition to D-II by 2027.

NCAA Division II is the smallest division in the NCAA by school count.

Division II institutions

Full members