Aunt Flo is a popular euphemism referring to menstruation. It may also refer to:
Characters
A character in the British television series ...And Mother Makes Three A character in the children's television series Bod A character in the 1984 short story "Gramma" by Stephen King A character in the television series The Mary Tyler Moore Show A character in the 2007 book Pontoon: A Novel of Lake Wobegon by Garrison Keillor A character in the animated series South Park A character in the American sketch comedy television series Wonder Showzen