Thou Shalt Not may refer to:

"Thou shalt not", the initial phrase used in the King James Version of the Bible for most of the Ten Commandments ThouShaltNot, a former music band Thou Shalt Not (musical), a Broadway musical based on the novel Thérèse Raquin by Émile Zola Thou Shalt Not (album), a cast recording "Thou Shalt Not...", a 1989 episode of television series Quantum Leap Thou Shalt Not (film), a 1919 American drama film directed by Charles Brabin "Thou Shalt Not", 1940 photograph by Whitey Schafer