Route 27, or Highway 27, may refer to:
Australia Burke Developmental Road (Queensland) Zeehan Highway (Tasmania)
Canada Alberta Highway 27 British Columbia Highway 27 Manitoba Highway 27 Prince Edward Island Route 27 Saskatchewan Highway 27
Ontario Ontario Highway 27 York Regional Road 27, formerly Highway 27 in York Region Simcoe County Road 27, formerly Highway 27 in Simcoe County
Chile Chile Route 27
Costa Rica National Route 27
Croatia D27 road (Croatia)
Czech Republic part of I/27 Highway; Czech: :cz:Silnice I/27
France A27 autoroute Route nationale 27
Germany Bundesautobahn 27 Bundesstraße 27
Greece A27 motorway, from Kozani to Ptolemaida and from Florina to Niki A27 road, a limited-access road from Ptolemaida to Florina EO27 road, from Amfissa to Bralos
India National Highway 27 (India)
Iran
Ireland N27 road (Ireland)
Italy Autostrada A27
Japan Japan National Route 27 Maizuru-Wakasa Expressway
Korea, South Suncheon–Wanju Expressway National Route 27
Malaysia
KLIA Outer Ring Road
Montenegro R-27 regional road (Montenegro)
Netherlands A27 motorway (Netherlands)
New Zealand New Zealand State Highway 27
Norway Norwegian County Road 27
Russia M27 highway (Russia)
Spain Autovía A-27
Switzerland European route E27 (partly also in France and Italy)
United Kingdom British A27 (Whiteparish-Pevensey) M27 (Cadnam-Portsmouth)
United States Interstate 27 U.S. Route 27 Alabama State Route 27 County Route 27 (Lee County, Alabama) Arkansas Highway 27 Arkansas Highway 27B Arkansas Highway 27N (former) California State Route 27 County Route A27 (California) County Route J27 (California) County Route S27 (California) Connecticut Route 27 Florida State Road 27 Georgia State Route 27 Idaho State Highway 27 Illinois Route 27 (former) Iowa Highway 27 K-27 (Kansas highway) Louisiana Highway 27 Maine State Route 27 Maryland Route 27 Maryland Route 27B Maryland Route 27C Maryland Route 27D Maryland Route 27E Massachusetts Route 27 M-27 (Michigan highway) Minnesota State Highway 27 County Road 27 (Hennepin County, Minnesota) County Road 27 (Ramsey County, Minnesota) County Road 27 (Scott County, Minnesota) County Road 27 (Washington County, Minnesota) Mississippi Highway 27 Missouri Route 27 Missouri Route 27 (1922) (former) Nebraska Highway 27 Nebraska Spur 27D Nebraska Recreation Road 27B Nebraska Recreation Road 27C Nevada State Route 27 (former) New Hampshire Route 27 New Jersey Route 27 County Route 27 (Bergen County, New Jersey) County Route 27 (Monmouth County, New Jersey) County Route 27 (Ocean County, New Jersey) New Mexico State Road 27 New York State Route 27 County Route 27 (Allegany County, New York) County Route 27 (Cattaraugus County, New York) County Route 27 (Clinton County, New York) County Route 27 (Columbia County, New York) County Route 27 (Dutchess County, New York) County Route 27 (Erie County, New York) County Route 27 (Greene County, New York) County Route 27 (Lewis County, New York) County Route 27 (Livingston County, New York) County Route 27B (Livingston County, New York) County Route 27 (Nassau County, New York) County Route 27 (Ontario County, New York) County Route 27 (Oswego County, New York) County Route 27 (Rensselaer County, New York) County Route 27 (Rockland County, New York) County Route 27 (Schoharie County, New York) County Route 27 (Schuyler County, New York) County Route 27 (St. Lawrence County, New York) County Route 27B (Suffolk County, New York) (former) County Route 27 (Ulster County, New York) County Route 27 (Washington County, New York) County Route 27 (Westchester County, New York) County Route 27 (Wyoming County, New York) County Route 27 (Yates County, New York) North Carolina Highway 27 North Dakota Highway 27 Ohio State Route 27 (1923-1927) (former) Oklahoma State Highway 27 Oregon Route 27 Pennsylvania Route 27 South Carolina Highway 27 South Dakota Highway 27 Tennessee State Route 27 Texas State Highway 27 Farm to Market Road 27 Texas Park Road 27 Utah State Route 27 Virginia State Route 27 Virginia State Route 27 (1918-1933) (former) Virginia State Route 27 (1940–1953) (former) Washington State Route 27 West Virginia Route 27 Wisconsin Highway 27
Territories Guam Highway 27 Puerto Rico Highway 27
See also List of highways numbered 27A