The 2000 Eastern Washington Eagles football team represented Eastern Washington University as a member of the Big Sky Conference during the 2000 NCAA Division I-AA football season. Led by first-year head coach Paul Wulff, the Eagles compiled an overall record of 6–5, with a mark of 5–3 in conference play, and finished tied for second in the Big Sky. The team played home games at Woodward Field in Cheney and Joe Albi Stadium in Spokane, Washington.

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