The 1940 Case Rough Riders football team represented the Case School of Applied Science—later merged into Case Western Reserve University—as a member of the Big Four Conference and the Ohio Athletic Conference (OAC) during the 1940 college football season. Led by 11th-year head coach Ray A. Ride, the Rough Riders compiling an overall record of 6–2. Case played second in the Big Four with a record of 2–1 and third in the OAC with a mark of 3–0. The team played home games at Shaw Stadium in Cleveland.

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