Human chromosome X open reading frame 58 (CXorf58) is a protein coding gene encoding the CXorf58 protein.
Gene CXorf58 contains 9 exons. CXorf58 is 31,578 nucleotides long. The longest exon in CXorf58 is exon 1, containing 324 nucleotides and the shortest exon is exon 4, containing 111 nucleotides. CXorf58 is highly expressed in sperm cells, particularly at the lower point of the head and midpiece of spermatids.
Isoforms There are two isoforms for CXorf58 protein. Isoform 1 for CXorf58 is 332 amino acids long and isoform 2 is 330 amino acids long. Isoform 2 uses an alternate in-frame splice site in the 3' coding region, resulting in a shorter protein.
Protein
The molecular weight of CXorf58 isoform 1 protein is 38.9kDa. It is rich in basic amino acids and abundant in the amino acids methionine and arginine. CXorf58 contains 3 asparagine-linked glycosylation sites and 5 phosphorylation sites.
Gene level regulation CXorf58 contains a tissue-specific expression pattern. It is found in high abundance in the testis.
Protein level regulation CXorf58 is localized in the cytoplasm of cells. CXorf58 contains 10 net phosphorylation sites. There is one glycosylation site for CXorf58 at amino acid 320.
Homology Orthologs of CXorf58 appear in an array of taxonomic groups. +SpeciesCommon nameNCBI Accession NumberSequence length (amino acids)Date of Divergence (MYA)Sequence identity (%)Sequence similarity (%)Homo sapiensHumanXP_054182731.1332 - - -Gorilla gorilla gorillaWestern lowland gorillaXP_004063966.23328.699%99%Sciurus carolinensisEastern gray squirrelXP_047392635.13708762%69%Mus MusculusHouse mouseNP_001349810.13688756%65%Equus quaggaPlains zebraXP_046529678.13749464%71%Panthera unciaSnow leopardXP_049499554.13809464%66%Cervus canadensisElkXP_043314584.13729463%69%Bos taurusCattleXP_024843724.13709463%68%Struthio camelus australisSouth African ostrichXP_009663695.134231949%54%Dromaius novaehollandiaeEmuXP_025959174.133431948%53%Nothoprocta perdicariaChilean tinamouXP_025894453.133231947%51%Chrysemys picta belliiPainted turtleXP_065435515.140831948%53%Chelonia mydasGreen sea turtleXP_037757767.135331948%54%Danio rerioZebrafishXP_021332053.125342944%65%Asterias rubensCommon starfishXP_033637843.140461944%53%Saccoglossus kowalevskiiAcorn wormXP_002736551.139061944%53%Ruditapes philippinarumManila clamXP_060606373.144668645%42%Lineus longissimusBootlace wormXP_064639061.139368645%52%Liolophura japonicaChiton liolophura japonicaXP_064615141.143568645%45%Patella vulgataCommon limpetXP_050389200.143468641%45%
Interacting proteins Five proteins have interactions with CXorf58: GDE1, H2BC9, HOXA1, TGFBR2, and DCAF5. +ProteinProtein Full NameFunctionGDE1Glycerophosphodiester Phosphodiesterase 1Enables glycerophosphodiester phosphodiesterase activity, glycerophosphoinositol glycerophosphodiesterase activity, and lysophospholipase activity.H2BC9H2B Clustered Histone 9Encodes a replication-dependent histone that is a member of the histone H2B family.HOXA1Homeobox A1Encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.TGFBR2Transforming Growth Factor Beta Receptor 2Forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta.DCAF5DDB1 and CUL4 Associated Factor 5Involved in protein ubiquitination.
Clinical significance Mutations that occur on CXorf58 include deletions of base pairs, missense mutations, nonsense mutations, and frameshift mutations. Many of which are coding sequence variants.
References