Ussing is a surname. Notable people with the surname include:
Elisa Ussing (1885–1949), Danish lawyer Gregers Algreen-Ussing (born 1938), Danish architect Hans Ussing (1911–2000), Danish scientist Ussing chamber, apparatus for measuring epithelial membrane properties Niels Viggo Ussing (1864–1911), Danish mineralogist Olaf Ussing (1907–1990), Danish film actor Susanne Ussing (1940–1998), Danish artist