Studio Uno ("Studio One") was an Italian Saturday night variety show, broadcast on Rai 1 (at the time Programma Nazionale) between 1961 and 1966. It is considered one of the most innovative shows on Italian television at the time.Grasso, Aldo; Scaglioni, Massimo. "Studio Uno". Enciclopedia della Televisione. Garzanti, 1996 – 2003. p. 308. . This show marked the debut of Mina as television presenter, as well as the breakout of Kessler Twins and Don Lurio.

Created and directed by Antonello Falqui, it was conceived by Falqui during a trip to the United States he made with the then RAI president Sergio Pugliese, and was inspired by Broadway theatre and by Fred Astaire and Ginger Rogers' musical films. For this reason, together with Don Lurio and a young Gino Landi, longtime Fred Astaire collaborator Hermes Pan was chosen as choreographer. The show has been described as 'characterized by a perfect equilibrium between comical sketches, songs, ballets and guests'. During the show Kesslers and Don Lurio launched the song "Da-da-un-pa". Bruno Canfora served as conductor in all the editions of the show.

C'era una volta Studio Uno ("Once upon a time Studio Uno"), a television miniseries inspired by the show, was broadcast in 2017; it stars Alessandra Mastronardi, Giusy Buscemi, Diana Del Bufalo and Edoardo Pesce as Falqui.

Editions Year Episodes Broadcasting dates Writers Cast 1961–196212 21 October – 13 January Guido Sacerdote, Dino Verde Mina, Marcel Amont, Kessler Twins, Bluebell Girls, Don Lurio, Quartetto Cetra, Mac Ronay, Renata Mauro, Emilio Pericoli 1962–196312 22 December – 16 March Antonello Falqui, Guido Sacerdote, Dino Verde Walter Chiari, Zizi Jeanmaire, Quartetto Cetra, Don Lurio, Bluebell Girls, Rita Pavone, Giancarlo Cobelli, Dany Saval 196512 13 February – 1 May Antonello Falqui, Guido Sacerdote, Castellano e Pipolo Mina, Luciano Salce, Lelio Luttazzi, Paolo Panelli, Milly, Kessler Twins 196620 12 February – 25 June Antonello Falqui, Guido Sacerdote, Lina Wertmüller Lelio Luttazzi, Sandra Milo, Luciano Salce, Ornella Vanoni, Rita Pavone, Kessler Twins, Mina, Romolo Valli, Franca Valeri, Caterina Caselli

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