The 1965 Texas Southern Tigers football team was an American football team that represented Texas Southern University as a member of the Southwestern Athletic Conference (SWAC) during the 1965 NAIA football season. Led by first-year head coach Clifford Paul, the Tigers compiled an overall record of 5–4–1, with a mark of 4–2–1 in conference play, and finished tied for second in the SWAC.

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