Iota Phi Theta is an historically African American fraternity founded in 1963 at Morgan State University in Maryland. Following is a list of collegiate and alumni chapters of Iota Phi Theta. Its chapters start as colonies and are assigned a Greek letter chapter designation after demonstrating viability for one year.

Collegiate chapters In the following list of collegiate chapters, active chapters are indicated in bold and inactive chapters are in italic.

ChapterCharter date and rangeInstitutionCity or countyState or countryStatusAlphaMorgan State UniversityBaltimoreMarylandActiveBeta Hampton UniversityHamptonVirginiaInactiveGamma1967Delaware State UniversityDoverDelawareActiveDelta1968Norfolk State UniversityNorfolkVirginiaActiveEpsilon1968New Jersey City UniversityJersey CityNew JerseyActiveZeta1969–xxxx ?, 2022North Carolina A&T State UniversityGreensboroNorth CarolinaActiveEtaVirginia State UniversityEttrickVirginiaActiveThetaUniversity of the District of ColumbiaWashingtonDistrict of ColumbiaActiveIota1971–xxxx ?,