Asselstine is a surname. Notable people with the surname include:
Brian Asselstine (born in 1953), American baseball player Jack Asselstine (1895–1966), Canadian ice hockey player Ron Asselstine (born 1946), Canadian ice hockey player William James Asselstine (1891–1973), Canadian politician
See also Asselstine, Ontario, communities of Loyalist, Ontario