Muffett is a surname. Notable people with the surname include:
Alec Muffett (born 1968), Anglo-American software engineer Billy Muffett (1930-2008), American baseball player
See also Little Miss Muffet, a nursery rhyme Thomas Muffet (1553-1604), English naturalist and physician Muffet, a spider-like character from the 2015 video game Undertale