Route 25 Business or Highway 25 Business may refer to:
Interstate 25 Business U.S. Route 25 Business Arkansas Highway 25B Maine State Route 25 Business Mississippi Highway 25 Business New York State Route 25A Business (former)
See also List of highways numbered 25 List of highways numbered 25A List of highways numbered 25AD List of highways numbered 25B List of highways numbered 25C List of highways numbered 25T