Ghiselin is a surname. Notable people with the surname include:
Brewster Ghiselin (1903–2002), American poet and academic Johannes Ghiselin (fl. 1491–1507), Flemish composer Michael Ghiselin (born 1939), American biologist, philosopher, and historian of biology Ogier Ghiselin de Busbecq (1522–1592), Flemish writer, herbalist and diplomat