Spoo may refer to:
Bob Spoo (1937–2018), American college football coach Caspar Mathias Spoo (1837–1914), Luxembourgish industrialist and politician Robert Spoo (born 1957), American legal scholar and educator Spoo (food), a fictional food in the Babylon 5 science fiction television series
See also Spoo Ness, a land feature in Unst Island, Shetland Islands - see North Sea#Extent