Sestini is a surname. Notable people with the surname include:
Andrea Sestini Hlaváčková (born 1986), Czech tennis player Bartolomeo Sestini (1792-1822), Italian poet Benedict Sestini (1816–1890), astronomer, mathematician and architect Giovanna Sestini (1749–1814), Italian operatic soprano Aldo Sestini, Italian geographer Giuliano Sestini (1931–1996), Italian Geologist