Lithurginae is a subfamily of woodborer bees in the family Megachilidae.

Genera These extant genera belong to the subfamily Lithurginae: Lithurgini Austrothurgus Lithurgopsis b (northern cactus woodborers) Lithurgus Berthold, 1827 i c g b Microthurge Michener, 1983 i c g Trichothurgus Moure, 1949 i c g Data sources: i = ITIS, c = Catalogue of Life, g = GBIF, b = Bugguide.net

The extinct genus Protolithurgus Engel, 2001 is placed in its own tribe Protolithurgiini, sister to Lithurgini within Lithurginae.

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