Gladney is a surname. Notable people with the surname include:
Bob Gladney (born 1957), Canadian ice hockey player Dru C. Gladney, American anthropologist Edna Gladney (1886–1961), American activist Graves Gladney (1907–1976), American illustrator Heather Gladney (born 1957), American writer Jeff Gladney (1996–2022), American football player Larry Gladney (born 1957), American physicist and cosmologist Lynn Gladney, American politician