Ossorio is a surname. Notable people with the surname include:
Alfonso A. Ossorio (1916–1990), Filipino American artist Amando de Ossorio (1918–2001), Spanish film director Ángel Ossorio y Gallardo (1873-1946), Spanish lawyer and statesman Aníbal González Álvarez-Ossorio (1876–1929), Spanish architect Beatriz de Bobadilla y Ossorio (1462–1501), ruler of La Gomera Constanza Ossorio (1595–1637), Spanish poet and writer Filippo Alferio Ossorio (1634–1693), Italian Catholic prelate Peter G. Ossorio (1926–2007), American psychologist