Chromopertubation is a method for the study of fallopian tube patency (a state of being open or unobstructed) for suspected infertility in women caused by fallopian tube obstruction. Occlusion or pathology of the fallopian tubes is the most common cause of suspected infertility. Chromopertubation is sometimes commonly referred to a "laparoscopy and dye" test. It is currently one of the standard procedures in this field. In most cases, chromopertubation is performed to assess and determine the cause of someone's difficulties in getting pregnant.
Procedure Chromopertubation is a medical procedure in which blue dye is injected into the fallopian tubes to detect if there are any blockages. It is performed as a laparoscopy, a minimally invasive procedure with small incisions. A laparoscope, a long, fine instrument is inserted into the abdomen close to the umbilicus in order to see one's internal organs, in particular the fallopian tubes. There is also a device called a uterine manipulator that is placed through the vagina and cervix into the uterus. As part of a laparoscopy, a blue dye solution (methylene blue or indigo carmine) is introduced into the uterine cavity. The dye solution will help determine if the fallopian tubes are open or blocked. If the fallopian tubes are open, the dye solution will enter and drain out into the pelvic cavity through the ends of the tubes. A tubular blockage is suspected if the fluid does not enter into the fallopian tubes or enters, but does not drain out of the fallopian tubes. Blocked fallopian tubes can be a factor of infertility in which the fallopian tubes will not allow the egg and sperm to meet.
Advantages Chromopertubation with laparoscopy is considered the "gold standard" to evaluate tubal patency. It is the most accurate way to look at the abdominal cavity and other pelvic structures. Other problems that can be viewed during the procedure are malformations of the uterus, adhesions, blocked fallopian tubes, or endometriosis. Slight adhesions inside a fallopian tube can be observed by the flow of dye solution and removed during the procedure. Other findings, such as endometriotic lesions, may also be treated as part of laparoscopy. If laparoscopy with chromopertubation shows a mild blockage within one or both of the fallopian tubes, surgical reconstruction of the tubes can be performed. Studies have shown that tubal flushing with a contrast medium could be used as a treatment for infertility, as it was noted that many women were able to conceive within the first 3–6 months after the procedure.
Contrast Medium Oil-based contrast medium is typically used in hysterosalpingography (HSG), while water-based contrast medium is regularly used in hystero contrast sonography (HyCoSy), hysterosalpingo-foam sonography (HyFoSy), laparoscopy with chromopertubation, and HSG. A meta-analysis that combined the results of five studies found that tubal flushing in HSG with an oil-based contrast medium moderately increased the odds of having a live-birth within six months. The benefit of a water-soluble contrast medium in tubal flushing is unclear. The effects of tubal flushing with either type of contrast media beyond six months are unknown and more research is needed to see if there is a difference in contrast media when used for tubal flushing in laparoscopy with chromopertubation.
Disadvantages Chromopertubation is done during laparoscopy, which requires general anesthesia. General anesthesia is usually safe, but problems, such as pain, nausea and vomiting, sore throat, and muscle aches may arise. Around the small incisions on the abdomen, there will be some pain and may also be some bruising around the lower abdomen. There is a risk of procedural complications, such as bleeding or abdominal injury, which may result in longer recovery times. Also, some bleeding in the vagina can be from the placement of the instrument during the dye test. Minor bleeds should stop within a couple of days. Another issue is the chance of cornual spasms, which often occur if the injection of dye is done too quickly. These spasms, or contractions, can block off the interstitial region (proximal portion) of the fallopian tubes, leading to an incorrect diagnosis of proximal tube occlusion. Furthermore, the insertion of the instruments into the abdomen can potentially injure major abdominal organs, such as the small and large intestines, bladder, and blood vessels. Previous surgeries or procedures, such as Caesarean surgery, ovarian cyst removal, bladder surgery, or appendix removal can increase the chances of abdominal organs damage during chromopertubation. Difficulties with the insertion of the laparoscope can rarely happen. These difficulties usually occur in those who are overweight or who have had previous abdomen scarring from another procedure. If the instrument cannot be inserted into the abdominal cavity, chromopertubation will not be recommended. Instead, alternative methods of checking the fallopian tubes will be taken into consideration. Because laparoscopy with chromopertubation is a more time intensive diagnostic procedure, if no significant findings are found, then there may be a delay in starting other infertility therapies.
Methylene blue dye Allergic-like reactions and methemoglobinemia have been documented after the use of methylene blue dye in chromopertubation. These reported symptoms vary from blue discoloration of body fluids to anaphylactic shock. Methemoglobinemia is a blood disorder that can potentially result in various levels of cyanosis. High levels of methylene blue dye can also result in hemolysis. In particular, methylene blue dye should be avoided in people using serotonergic drugs, such as selective serotonin reuptake inhibitors (SSRIs) and antidepressants, since this interaction may cause fatal serotonergic syndrome. Serotonergic syndrome presents as changes in mental status, in addition to overactivation of the neuromuscular and nervous systems. Common symptoms of fatal serotonergic syndrome include high fevers, seizures, and tremors. Other reported features of fatal serotonergic syndrome include hyperthermia, respiratory failure, rhabdomyolysis, metabolic acidosis, myoglobinuria, renal failure, coma, and death.
Oil-based contrast medium While there have been recent studies showing an increased odds of clinical pregnancy when an oil-based contrast medium is used for tubal flushing, there is also an increased risk for intravasation. Intravasation of oil-based contrast is the process of small oil droplets entering the blood circulation and causing inflammation of blood vessels or blocking blood vessels, potentially in the lungs or brain. Oil-based contrast medium also has a higher amount of iodine, as compared to water-based contrast medium, and this higher iodine exposure could lead to subclinical hypothyroidism.
Cost of procedure The cost of fallopian tube surgeries can range from $3,000 to up to about $13,000. The costs of the procedures and surgeries depends on insurance coverage, complications, and the type of fallopian tube surgery. In the female reproductive system, the fallopian tubes are one of the most important parts because the tubes connect to the ovaries where the eggs are released. To check if the fallopian tubes are blocked or damaged, a laparoscopy will cost from $1,700 to $5,000. If the fallopian tubes are blocked, there will be no fertilization and therefore, the egg and sperm cannot meet. If an individual wants to open up their fallopian tubes, a procedure called fallopian tube recanalization can be performed. During this procedure, cuts or incisions are not necessary, instead a catheter is inserted into the womb and fallopian tubes to make sure a blockage is confirmed, and then a second catheter is inserted to clear that blockage. This can cost about $3,000–$5,000. In addition, there is another procedure called salpingostomy when the fallopian tubes are filled with fluid. This procedure costs about $5,000–$7,000. The doctor opens the tube to remove the blockage, but does not disturb the position of the fallopian tube.
Alternatives There are also other procedures to determine whether or not someone's fallopian tubes are blocked. While laparoscopy with chromopertubation is considered to be the first-line practice for the diagnosis of tubal occlusion, it is generally a more expensive and invasive procedure, and it is recommended for women with comorbidities of both tubal occlusion and another pelvic pathology.
Hysterosalpingography Hysterosalpingography (HSG), a different diagnostic procedure for tubal occlusion, is commonly performed in women without suspected comorbidities. HSG is done initially with an X-ray examination performed by a radiologist. While the main purpose of HSG is to evaluate the fallopian tubes, HSG does not provide an explanation for why the tubes are blocked. The examination should be scheduled between 7–12 days of an individual's menstrual cycle. Ideally, it should be completed within 2–5 days after menses is over. Radiographic dye is used to assess the uterine cavity and fallopian tubes. It can be used to study suspected infertility, as well as tubal disease. During a HSG procedure, the uterus is injected with contrast dye, which can be observed via fluoroscopy. The test results are normal if the X-ray shows a normal uterine shape, and the dye spills out from the ends of the fallopian tubes. There may be a problem if the dye shows an abnormally shaped uterus, and the dye does not flow freely from the fallopian tubes. While this method can be completed relatively quickly since anesthesia is not needed, there is a risk of tubal spasm, which typically occurs during injection of the contrast dye due to increased pressure. With tubal spasm, the fallopian tubes can close, and this may give a false diagnosis of tubal occlusion. While both HSG and chromopertubation can cause spasms, it is more likely to happen with HSG. HSG also has the disadvantage of exposing the patient to radiation from the X-ray that is performed. The radiographic dye, which is iodine-based, can also cause allergic reactions. Though this generally does not occur, there is an increased risk of genital tract infections so patients may be prescribed antibiotics.
Sonohysterosalpingography Sonohysterosalpingography (SHG), a type of ultrasound exam, is another procedure that assesses patency through the use of varying contrast medium. One method is to introduce air into the uterine cavity and observe air bubbles in the fallopian tubes. The second method is to use distilled water or normal saline to observe the movement of fluid through the tubes. Here, fluid enters the uterus through the cervix via a plastic tube. The last method involves combining air with saline to make it easier to see on ultrasound. Images of the uterus lining are created through the use of sound waves, and the fluid introduced into the uterus serves to enhance the images. Side effects of this exam include cramping and a rare possibility of pelvic infection. Generally this procedure can be done quickly since no sedation is required and it tends to be a cheaper alternative compared to the other methods.
Hystero contrast sonography