Middlesex is a historic county in southeast England.
Middlesex may also refer to:
Places Australia Middlesex, Tasmania, a locality in Australia Middlesex, Western Australia, a locality of the Shire of Manjimup
Belize Middlesex, Belize, a village in Stann Creek District, Belize
Canada Middlesex County, Ontario Middlesex (federal electoral district) Middlesex (Province of Canada electoral district) Middlesex (provincial electoral district) Municipalities in Middlesex County: Middlesex Centre North Middlesex, Ontario Southwest Middlesex, Ontario
Jamaica Middlesex County, Jamaica
United Kingdom Middlesex (UK Parliament constituency)
United States Middlesex, New Jersey Middlesex, New York Middlesex, North Carolina Middlesex, Vermont Middlesex, Virginia Middlesex Township, Butler County, Pennsylvania Middlesex Township, Cumberland County, Pennsylvania Middlesex County, Connecticut Middlesex County, Massachusetts Middlesex County, New Jersey Middlesex County, Virginia West Middlesex, Pennsylvania, a borough in Mercer County
Literature Middlesex (novel), by Jeffrey Eugenides
Other Middlesex (1783 EIC ship), an East Indiaman of the British East India Company Middlesex Canal, Massachusetts Middlesex County Cricket Club, St John's Wood, London Middlesex Guildhall, Westminster, London Middlesex Middle School, Connecticut Middlesex Plat Historic District, listed on the National Register of Historic Places in Polk County, Iowa Middlesex School, Concord, Massachusetts Middlesex University, based in Hendon, London North Middlesex University Hospital, Edmonton, London West Middlesex University Hospital, Isleworth, London