Twomile Creek may refer to:
Twomile Creek (Chattahoochee River tributary), a stream in Georgia Twomile Creek (Marmaton River), a stream in Missouri Twomile Creek (North Fork Salt River), a stream in Missouri Twomile Creek (Warm Fork Spring River), a stream in Missouri Twomile Creek (Uwharrie River tributary), a stream in Randolph County, North Carolina Twomile Creek (East Branch Oil Creek tributary), a stream in Crawford County, Pennsylvania Twomile Creek (Kanawha River), a stream in West Virginia Twomile Creek (Nepco Lake tributary), a stream in Wisconsin Two Mile Creek (Yellow River tributary), a stream in Wisconsin