The women's 100 metre breaststroke SB9 event at the 2016 Paralympic Games took place on 8 September 2016, at the Olympic Aquatics Stadium. Two heats were held. The swimmers with the eight fastest times advanced to the final.

Heats Heat 1 10:24 8 September 2016:

Rank Lane Name Nationality Time Notes 1 5 1:20.84 2 3 1:21.67 3 4 1:21.97 4 6 1:27.08 5 2 1:27.47

Heat 2 10:28 8 September 2016: Rank Lane Name Nationality Time Notes 1 5 1:15.47 2 4 1:17.38 3 3 1:19.51 4 6 1:20.99 5 2 1:27.15

Final 18:12 8 September 2016:

Rank Lane Name Nationality Time Notes 4 1:15.49 1 1:16.87 5 1:17.01 4 3 1:17.93 5 6 1:19.46 6 7 1:20.44 7 2 1:20.90 8 8 1:25.74

Notes