The 2016 North Alabama Lions football team represented the University of North Alabama as a member of the Gulf South Conference (GSC) during the 2016 NCAA Division II football season. Led by Bobby Wallace in 15th and final season as head coach, the Lions compiled an overall record of 11–2 with a mark of 7–0 in conference play, winning the GCS title. North Alabama advanced to the NCAA Division II football championship playoffs, where, after the first-round bye, the Lions defeated in the second round, in the quarterfinals, and Shepherd in the semifinals before losing to Northwest Missouri State in the NCAA Division II Championship Game. The team played home games at Braly Municipal Stadium in Florence, Alabama.

Schedule North Alabama announced its 2016 football schedule on February 9, 2016. The schedule consists of five home games and five away games in the regular season.

The game between Florida Tech and North Alabama was cancelled in advance of the arrival of Hurricane Matthew.

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