Biot is a French surname. It may refer to
Camille Biot (1850–1918), French physician Charlie Biot (1917–2000), American Negro League baseball player Édouard Biot (1803–1850), French engineer and sinologist Jacques Biot (born 1952), French engineer, businessman and academic administrator Jean-Baptiste Biot (1774–1862), French physicist and mathematician Maurice Anthony Biot (1905–1985), Belgian-American physicist