Girolami is an Italian surname. Notable people with the surname include:
Ennio Girolami (1935–2013), Italian actor Gregory S. Girolami, American professor of chemistry Girolami method Marino Girolami (1914–1994), Italian film director Mark Girolami (born 1963), British statistician and engineer Néstor Girolami (born 1989), Argentine racing driver Paul Girolami (1926–2023), British-Italian businessman Remigio dei Girolami (1235–1319), Italian Dominican theologian