The following railroads operate in the U.S. state of Ohio.
Common freight carriers Akron Barberton Cluster Railway (AB) Ann Arbor Railroad (AA), (Watco) Ashland Railway (ASRY) Ashtabula, Carson and Jefferson Railroad (ACJR) Belpre Industrial, Parkersburg Railroad (BIP) Camp Chase Railway (CAMY) Canadian National Railway (CN) through subsidiaries Bessemer and Lake Erie Railroad (BLE) and Grand Trunk Western Railroad (GTW) Central Railroad of Indiana (CIND) (owned by Genesee & Wyoming (GWI)) Chicago, Fort Wayne and Eastern Railroad (CFE) (owned by GWI) Operates Delphos Terminal Cincinnati East Terminal Railway (CET) Cleveland Commercial Railroad (CCRL) Cleveland Harbor Belt Railroad (CHB) Cleveland Works Railway (CWRO) Columbus and Ohio River Rail Road (CUOH) (owned by GWI) CSX Transportation (CSXT) including subsidiary Three Rivers Railway Flats Industrial Railroad (FIR) Grand River Railway (GRRY) Indiana Eastern Railroad (IERR) Indiana Northeastern Railroad (IN) Indiana and Ohio Railway (IORY) (owned by GWI) Operates South Charleston Railroad, West Central Ohio Port Authority Kanawha River Railroad (KNWA) (Watco) Lake Terminal Railroad (LT) Lorain Northern Railroad (LNOR) Mahoning Valley Railway (MVRY) (owned by GWI) Napoleon, Defiance & Western Railroad (NDW), (Pioneer Lines) Newburgh and South Shore Railroad (NSR) (Omnitrax) Norfolk Southern Railway (NS) including subsidiary Cincinnati, New Orleans and Texas Pacific Railway (CNTP) Northern Ohio and Western Railway (NOW) Ohio and National Railway Company (ONRR) Ohi-Rail Corporation (OHIC) (taken over by Mahoning Valley Railway (MVRY)) Ohio Central Railroad (OHCR) (owned by GWI) Ohio South Central Railroad (OSCR) Ohio Southern Railroad (OSRR) (owned by GWI) Ohio Terminal Railway (OHIO) Republic N&T Railroad (NTRY) R.J. Corman Railroad/Cleveland Line (RJCL) R.J. Corman Railroad/Western Ohio Lines (RJCW) RSL Railroad (RSL) Warren and Trumbull Railroad (WTRM) (owned by GWI) Wheeling and Lake Erie Railway (WE) Youngstown and Austintown Railroad (YARR) (owned by GWI) Youngstown Belt Railroad (YB) (owned by GWI) Youngstown and Southeastern Railroad (YSRR) Operates Eastern States Railroad and Ohio and Pennsylvania Railroad (OHPA)
Private freight carriers Name Reporting Mark AK Steel AKS Railserve RSSX Shelly Materials SHIXTemperance Yard Corporation TYCX U.S. Rail Corporation USRC Centrus NULL Dupps Company DUKX
Passenger carriers
Name Reporting Mark Type Amtrak AMTK Intercity Cincinnati Bell Connector NULL Rapid transit Cuyahoga Valley Scenic Railroad CVSR Tourist Hocking Valley Scenic Railway HVSR Tourist Lake Shore Railway Association NULL Tourist Lebanon Mason Monroe Railroad CRC Tourist RTA Rapid Transit RTA Rapid transit Toledo, Lake Erie and Western Railway TLEW Tourist Zanesville & Western Scenic Railroad ZWSX Tourist
Defunct railroads