Tennis singles was an event at the 2014 Knoxville Challenger. It was the eleventh iteration of the annual Knoxville Challenger professional tennis tournament. Tim Smyczek was the defending champion. He lost to Sam Groth in the semifinals. Adrian Mannarino won the tournament, defeating Sam Groth 3–6, 7–6(8–6). 6–4 in the final.
Seeds
Draw
Finals
Top half
Bottom half
References Main draw Qualifying draw