Guido is a surname. Notable people with the surname include:
Alejandro Guido (born 1994), American soccer player Beatriz Guido (1924–1988), Argentine novelist and screenwriter Guilherme Guido (born 1987), Brazilian swimmer José María Guido (1910–1975), interim President of Argentina from 1962 to 1963 Luigi Guido (born 1968), Italian judoka Marcello Guido (born 1953), Italian architect Margaret Guido (1912–1994), British archaeologist, prehistorian, and finds specialist Tomás Guido (1788–1866), general in the Argentine War of Independence, diplomat and politician