O-phospho-L-serine—tRNA ligase (, O-phosphoseryl-tRNA ligase, non-canonical O-phosphoseryl-tRNA synthetase, SepRS) is an enzyme with systematic name O-phospho-L-serine:tRNACys ligase (AMP-forming). This enzyme catalyses the following chemical reaction:
ATP + O-phospho-L-serine + tRNACys AMP + diphosphate + O-phospho-L-seryl-tRNACys
In organisms like Archaeoglobus fulgidus, this enzyme ligates O-phosphoserine to tRNACys.
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