(R)-amidase (, R-stereospecific amidase, R-amidase) is an enzyme with systematic name (R)-piperazine-2-carboxamide amidohydrolase. The enzyme characterised from Pseudomonas species catalyses several related hydrolysis reactions.
For example, it converts β-alaninamide to β-alanine:
It also acts on (R)-piperazine-2-carboxamide:
Other substrates include (R)-N-tert-butylpiperazine-2-carboxamide and (R)-piperidine-3-carboxamide. Similar amidases from other organisms are known, including one dependent on cobalt for activity. Immobilized enzyme has been used to produce single-enantiomer piperazine-2-carboxylic acid from the racemic amide.
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