Mazzotti is a surname. Notable people with the surname include:
Adriano Sauro Lorenzo Mazzotti (born 1966), director of South African company Carnilinx (Pty) Ltd. José Antonio Mazzotti, Peruvian poet, scholar, and literary activist Lisa Mazzotti (born 1965), Italian voice actor Pascal Mazzotti (1923–2002), French actor
See also Mazzotti Reaction, a symptom complex seen in tropical medicine