Exodeoxyribonuclease III (EC 3.1.11.2, Escherichia coli exonuclease III, E. coli exonuclease III, endoribonuclease III) is an enzyme that catalyses the following reaction

Exonucleolytic cleavage in the 3′- to 5′-direction to yield nucleoside 5′-phosphates

This enzyme has a preference for double-stranded DNA.

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