Maltokinase () is an enzyme with systematic name ATP:alpha-maltose 1-phosphotransferase. It catalyses the following chemical reaction

The enzyme characterised from the bacterium, Mycobacterium bovis, converts the disaccharide, maltose, to maltose 1-phosphate by transferring a phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP). The enzyme requires magnesium ions (Mg2+) for activity.

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