Neomycin C transaminase (, neoN (gene)) is an enzyme with systematic name 2-oxoglutarate:neomycin C aminotransferase. It catalyses the final step in the biosynthesis of neomycin C, which transfers an amino group from L-glutamic acid to the aldehyde, 6"'-oxoneomycin C, with α-ketoglutaric acid as a byproduct. The preceding two steps in the sequence are catalysed by 2"'-acetyl-6"'-hydroxyneomycin C deacetylase and 6"'-hydroxyneomycin C oxidase.
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