This is a list of railroads operating in the U.S. state of Florida.

Current railroads Common freight carriers Alabama and Gulf Coast Railway (AGR) (GWI) AN Railway (AN) (GWI) Bay Line Railroad (BAYL) (GWI) CSX Transportation (CSXT) First Coast Railroad (FCRD) (GWI) Florida Central Railroad (FCEN) Florida East Coast Railway (FEC) Florida Gulf & Atlantic Railroad (FGA) Florida Midland Railroad (FMID) Florida Northern Railroad (FNOR) Georgia and Florida Railway (GFRR) Jacksonville Port Terminal Railroad (JXPT) (Watco) Norfolk Southern Railway (NS) through subsidiary Georgia Southern and Florida Railway (GSF) Seminole Gulf Railway (SGLR) South Central Florida Express, Inc. (SCXF)

Private freight carriers CF Industries (CFIZ) Conrad Yelvington Distributors, Inc. (CNYX) The Mosaic Company (IMCX) Orlando Utilities Commission (OUCX) Perdue Farms (PFMX) Port Manatee Railroad (MAUP) Port of Palm Beach District (PPBD) Port of Panama City (PPC) United States Sugar Corporation (USSC)

Passenger carriers

Amtrak (AMTK) Brightline (BLFX) Florida Railroad Museum (FGCX) Gold Coast Railroad Museum (GCOX) Jacksonville Skyway Kirby Family Farm Train Miami Metromover and Miami MetrorailUnder same management and with a walking-distance transfer between the two. Seminole Gulf Railway (SGLR) Serengeti Express (located in Busch Gardens Tampa) Sugar Express (CHOX) SunRail (CFRC) Tradewinds & Atlantic Railroad TECO Line Streetcar Tri-Rail (TRCX) Walt Disney World Walt Disney World Monorail and Walt Disney World RailroadUnder same management and with a walking-distance transfer between the two. Wildlife Express Train

Private carriers Clay Springs and Apopka Railroad Ellaville, West Lake and Jennings Railroad Florida and Georgia Railway Peace River Phosphate Company Pensacola and Andalusia Railroad Starke and New River Railroad Union Cypress Company Winston Lumber Company

Never built Apopka and Atlantic Railroad Choctawhatchee and Northern Railroad Glen Cove Springs and Fort Fanning Railroad

Defunct railroads

NameMarkSystemThis is one or more of the Class I railroads that the railroad became part of, if any.FromToSuccessorNotesAlabama and Florida Railroad (1853–1869)L&N18531869Pensacola and Louisville RailroadAlabama and Florida Railroad (1898–1900)L&N18981900Louisville and Nashville RailroadAlabama and Florida Railroad (1936–1941)19361941Ceased operations in 1941Alabama and Florida Railroad (1986–1992)AFLR19861992Alabama and Florida RailwayAlabama and Florida RailwayAF19922011Ceased operations in 2011Alabama and Western Florida Railroad19261939Alabama, Florida and Gulf Railroad19171936Alabama and Florida Railroad (1936–1941)Alabama, Florida and Southern Railroad19101917Alabama, Florida and Gulf RailroadAlafia, Manatee, and Gulf Coast RailroadSAL18971906Charlotte Harbor and Northern RailwayAndalusia, Florida and Gulf Railway19191925N/AApalachicola Northern RailroadAN1903Later served by the AN Railway, the line is abandoned.Arcadia, Gulf Coast and Lakeland Railroad18911893Florida, Peninsular and Gulf RailroadArlington and Atlantic RailwayFEC18921892Jacksonville and Atlantic RailroadAtlanta and St. Andrews Bay RailwayA&SA, ASAB19061993Bay Line RailroadAtlantic Coast Line RailroadACLACL19021967Seaboard Coast Line RailroadAtlantic Coast, St. Johns and Indian River RailwayFEC18831899Southeastern RailwayAtlantic and East Coast Terminal CompanyACL/ FEC1905Atlantic and Gulf RailroadA&GACL18661879Savannah, Florida and Western RailwayAtlantic, Gulf and West India Transit CompanySAL18721881Florida Transit RailroadAtlantic, Suwannee River and Gulf RailroadSAL18931893Atlantic, Suwannee River and Gulf RailwayAtlantic, Suwannee River and Gulf RailwaySAL18931909Seaboard Air Line RailwayAtlantic, Valdosta and Western RailwaySOU18971902Georgia Southern and Florida Railway, St. Johns River Terminal CompanyAtlantic and Western RailroadFEC18881896Florida East Coast RailwayAugusta, Tallahassee and Gulf RailroadSAL18881891Carrabelle, Tallahassee and Georgia RailroadBirmingham, Columbus and St. Andrews Railroad19031926Alabama and Western Florida RailroadBlue Spring, Orange City and Atlantic RailroadFEC18781887Atlantic and Western RailroadBradford Farms RailroadPrison Spur between Raiford and LawteyBrooksville and Hudson Railroad 1902 1907 Tampa Northern RailroadBrooksville and Inverness RailwaySAL19251946Seaboard Air Line RailroadBurlington Northern Inc.BN19801981Burlington Northern RailroadBurlington Northern RailroadBN19811996Burlington Northern and Santa Fe RailwayBurlington Northern and Santa Fe RailwayBNSF19961997Alabama and Gulf Coast RailwayCarrabelle, Tallahassee and Georgia RailroadSAL18911906Georgia, Florida and Alabama RailwayCentral of Georgia RailwayCGCG19041939N/ACharlotte Harbor and Northern RailwaySAL19061946Seaboard Air Line RailroadChattahoochee and East Pass RailwayACL18811884Savannah, Florida and Western RailwayDeLand and St. John's River RailroadACL18861890Jacksonville, Tampa and Key West RailwayEast Florida RailwayACL18801884Savannah, Florida and Western RailwayEast Florida and Atlantic RailroadSAL18911893Florida Central and Peninsular RailroadEast and West Coast RailwayE&WCSAL19131945Seaboard Air Line RailroadFellsmere Railroad19111924Trans-Florida Central RailroadFernandina and Amelia Beach RailwaySAL18831894Florida Central and Peninsular RailroadFernandina and Jacksonville RailroadSAL18801884Florida Railway and Navigation CompanyFlorida RailroadSAL18531872Atlantic, Gulf and West India Transit CompanyFlorida Railway1905Florida Railway and Navigation CompanySAL18841888Florida Central and Peninsular RailroadFlorida and Alabama Railroadbefore 19141939N/AFlorida, Alabama and Gulf Railroadbefore 19111919Andalusia, Florida and Gulf RailwayFlorida, Atlantic and Gulf Central RailroadSAL18511868Florida Central RailroadFlorida Central RailroadACL19071914Atlantic Coast Line RailroadFlorida Central RailroadSAL18681882Florida Central and Western RailroadFlorida Central and Gulf RailwaySAL19161931N/AFlorida Central and Peninsular RailroadSAL18881903Seaboard Air Line RailwayFlorida Central and Western RailroadSAL18821884Florida Railway and Navigation CompanyFlorida Coast and Gulf RailwayFEC18921892Jacksonville, St. Augustine and Indian River RailwayFlorida, Georgia and Western RailwaySAL18911895Tallahassee Southeastern RailwayFlorida Midland RailwayACL18831896Atlantic Coast Line RailroadFlorida, Peninsular and Gulf Railroad1893Florida Southern RailroadACL18921903Atlantic Coast Line RailroadFlorida Southern RailwayACL18811892Florida Southern RailroadFlorida Steinhatchee RailwayFlorida Transit RailroadSAL18811883Florida Transit and Peninsular RailroadFlorida Transit and Peninsular RailroadSAL18831884Florida Railway and Navigation CompanyFlorida West Shore RailwaySAL19031909Seaboard Air Line RailwayFlorida Western RailroadSAL18811882Pensacola and Atlantic RailroadFlorida Western and Northern RailroadSAL19241945Seaboard Air Line RailroadFort Myers Southern RailroadFMSACL19171987Atlantic Coast Line RailroadFort White and Southern Railway1896Fort Wilderness RailroadFWRR19741980Gainesville and Gulf RailwaySAL18951907Tampa and Jacksonville RailwayGainesville, Ocala and Charlotte Harbor RailroadACL18761881Florida Southern RailwayGainesville, Rocky Point and Micanopy RailwaySAL18841895Gainesville and Gulf RailwayGeorgia and Florida RailNetGFRR19992005Georgia and Florida RailwayGeorgia and Florida RailroadGFRR19951999Georgia and Florida RailNetGeorgia and Florida RailroadG&FG&F19261951Valdosta Southern RailroadGeorgia and Florida RailwayG&FG&F19061926Georgia and Florida RailroadGeorgia, Florida and Alabama RailroadGF&ASAL19271974Seaboard Coast Line RailroadGeorgia, Florida and Alabama RailwayGF&ASAL19011928Georgia, Florida and Alabama RailroadGeorgia Pine RailwaySAL18951901Georgia, Florida and Alabama Railwayformer logging railroadGeorgia Southern and Florida RailroadSOU18891895Georgia Southern and Florida RailwayGreen Cove and Midland Railway18851892Southwestern RailroadGreen Cove Springs and Melrose Railroad18811885Green Cove and Midland RailwayGulf Coast Railway19151921N/AGulf, Florida and Alabama Railway