Nigerose phosphorylase (, cphy1874 (gene)) is an enzyme with systematic name 3-O-alpha-D-glucopyranosyl-D-glucopyranose:phosphate beta-D-glucosyltransferase. It catalyses the following chemical reaction:
The enzyme characterised from Clostridium phytofermentans is a type of glycoside hydrolase and is specific for nigerose (3-O-α-D-glucopyranosyl-D-glucopyranose), which it converts to D-glucose and β-D-glucose 1-phosphate, by adding the phosphate group (Pi) to one of the glucose units.
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