Mycothiol synthase (, MshD) is an enzyme with systematic name acetyl-CoA:desacetylmycothiol O-acetyltransferase. It catalyses the following chemical reaction:
This enzyme, found in Actinomycetota and characterised from Mycobacterium tuberculosis catalyses the last step in the biosynthesis of mycothiol. It is a member of a large family of GCN5-related N-acetyltransferases which transfer acetyl groups.
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