Spheroidene monooxygenase (, CrtA, acyclic carotenoid 2-ketolase, spirilloxantin monooxygenase, 2-oxo-spirilloxanthin monooxygenase) is an enzyme with systematic name spheroidene, reduced-ferredoxin:oxygen oxidoreductase (spheroiden-2-one-forming). This enzyme catalyses the following chemical reaction

spheroidene + reduced ferredoxin + O2 spheroiden-2-one + oxidized ferredoxin + H2O spirilloxantin + reduced ferredoxin + O2 2-oxospirilloxanthin + oxidized ferredoxin + H2O 2'-oxospirilloxanthin + reduced ferredoxin + O2 2,2'-dioxospirilloxanthin + oxidized ferredoxin + H2O

The enzyme is involved in spheroidenone biosynthesis and in 2,2'-dioxospirilloxanthin biosynthesis.

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